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한국식물학회 / v.48, no.2, 2005년, pp.258-262 
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( Genetic Transformation of Ajuga multiflora Bunge with Agrobacterium rhizogenes and 20-Hydroxyecdysone Production in Hairy Roots ) |
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| 초 록 |
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| An efficient transformation system for Ajuga multiflora Bunge was established by using Agrobacterium rhizogenes strain A4. After inoculation with the bacteria, we obtained a number of hairy-root clones from micro-calli of the explant petioles. One fast-growing line showed the highest production of 20-hydroxyecdysone (20-HE). PCR amplification of rooting locus (rol) genes revealed that the left hand-transferred DNA of the root-inducing plasmid was inserted into the genome of our transformed Ajuga hairy roots. This integration was further confirmed by DNA-DNA hybridization. The 20-HE content in hairy roots was 10 times higher than that measured in the wild type. |
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| 키워드 |
| Ajuga multiflora;hairy roots;20-hydroxyecdysone;Ri-plasmid;rol genes ; |
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Journal of Plant Biology / v.48, no.2, 2005년, pp.258-262
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200504704008670)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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