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Çѱ¹½Ä¹°ÇÐȸ / v.48, no.3, 2005³â, pp.263-269
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( Isolation and Characterization of Squalene Synthase cDNA from Centella asiatica (L.) Urban ) |
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We have cloned and characterized a gene for squalene synthase (SQS) from Centella asiatica (L.) Urban, a species that produces a large quantity of triterpene saponins such as asiaticoside and madecassoside. Its full-length cDNA clone was isolated by RACE PCR. The sequence of pSQS contains an open reading frame of 1248 nucleotides, which code for 416 amino acids with a molecular mass of 47.3 kDa. Southern analysis revealed that one copy might exist in the C. asiatica genome. We also determined that 0.1 mM methyl jasmonate was sufficient to up-regulate those levels of CaSQS mRNA. |
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Ű¿öµå |
asiaticoside;Centella asiatica;squalene synthase;triterpene saponins; |
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Journal of Plant Biology / v.48, no.3, 2005³â, pp.263-269
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200507521947923)
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³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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