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한국식물학회 / v.48, no.3, 2005년, pp.276-283 
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( Changes in Expression of the Cytosolic Ascorbate Peroxidase Gene, Ca-cAPX1, during Germination and Development of Hot Pepper Seedlings ) |
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| 초 록 |
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| Ascorbate peroxidase (APX), an ,antioxidant enzyme, scavenges $H_{2} O_{2}$ that is produced by normal metabolism and cellular oxidative stresses. To investigate its role during germination and seedling growth, we isolated a cDNA encoding cytosolic APX (cAPX) in hot pepper (Capsicum annuum L.). The full-length clone, Ca-cAPX1, is 1011 bp long and has an ORF encoding 249 amino acid residues. During seedling development, cAPX activity and expression levels were higher at Days 5 and 6 post-imbibition, respectively, whereas those of catalase (CAT) increased at Days 8 and 10. The increased amount of $H_{2} O_{2}$ in that early developmental stage (Day 5) may have been counteracted mainly by APX, and further removed by CAT in cooperation with APX. To determine whether the accumulation of $H_{2} O_{2}$ via suppression of cAPX expression might be a factor in stimulating germination, we constructed a transformant of Ca-cAPX1. Compared with the wild type, the germination rate for the antisense-suppressed Arabidopsis increased by $ 26% $, while its $H_{2} O_{2}$ content rose by $ 50% $. Therefore, we propose that the pre-germination suppression of cAPX expression stimulates seed germination by promoting the accumulation of $H_{2} O_{2}$. |
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| 키워드 |
| antisense suppression;ascorbate peroxidase;Capsicum annuum L.;hydrogen peroxide;seedling development; |
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Journal of Plant Biology / v.48, no.3, 2005년, pp.276-283
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200507521948035)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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