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한국식물학회 / v.48, no.4, 2005년, pp.380-386 
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( Growth Characteristics and Catalpol Production in Chinese Foxglove (Rehmannia glutinosa Liboschitz) Hairy Roots Transformed with Agrobacterium rhizogenes ATCC15834 ) |
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| 초 록 |
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| Hairy root clones of Rehmannia glutinosa were established via transformation with Agrobacterium rhizogenes ATCC15834. To optimize the culturing conditions for both root growth and catalpol production, effects of various combinations of seven basal media, pH, and carbon sources were examined under darkness. The fastest root growth was obtained in an SH medium containing $4%$ sucrose (pH 5.8); the highest catalpol content ($0.54%$ of dry weight) was achieved in a WPM medium supplemented with $4%$ sucrose (pH 5.8). Effects of plant growth regulators and chitosan were also investigated. IAA at $2mg L^{-1}$ significantly increased root lengths and the frequency of lateral roots. Chitosan $(50 mg L^{-1});and;GA_3 (0.5 mg L^{-1})$ induced catalpol production, with contents calculated at $0.7%$ dry weight and $0.65%$dry weight, respectively. |
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| 키워드 |
| catalpol;chitosan;hairy root cultures;PGRs;Rehmannia glutinosa; |
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Journal of Plant Biology / v.48, no.4, 2005년, pp.380-386
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200509408763829)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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