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Çѱ¹½Ä¹°ÇÐȸ / v.48, no.4, 2005³â, pp.404-410

( Effects on GSH Synthesis in Chinese Cabbage When the Culturing Solution Is Supplemented with Ammonium Sulfate or the Constituent Amino Acids for Glutathione )
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We examined the effects of the constituent amino acids for glutathione (GSH) -- glutamate (Glu), cysteine (Cys), and glycine (Gly) -- on GSH synthesis in Chinese cabbage seedlings. Glu, Cys, and Gly were applied simultaneously $(100 mg L^{-l})$ to the culture solution for 2d. When compared with the control, GSH concentrations were increased by 2.1fold $(640.4nmol g^{-l};FW)$ and 1.5-fold $(416.4 nmol g^{-l};FW)$ in the first leaf and the roots, respectively. Of all the free amino acids, the non-essentials, including Glu, Cys and Gly, occupied $95.5%$ (shoots) and $81.9%$ (roots) of the total. Cys supplements greatly enhanced the GSH concentration in the roots; application of $100 mg;L^{-l}$ increased the level by 7-fold over the control. The activity of GSH synthetase was higher in the roots than in the leaf, whereas that of $gamma¡©glutamylcysteine$ synthetase was higher in the leaf.
 
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chinese cabbage;cysteine;glutathione;glutathione synthetase;glutamylcysteine synthetase;
 
Journal of Plant Biology / v.48, no.4, 2005³â, pp.404-410
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ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200509408764028)
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