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Çѱ¹½Ä¹°ÇÐȸ / v.48, no.4, 2005³â, pp.440-447

( Expression of Tobacco cDNA Encoding Phytochelatin Synthase Promotes Tolerance to and Accumulation of Cd and As in Saccharomyces cerevisiae )
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The first tobacco cDNA encoding phytochelatin synthase (NtPCS1) has been cloned by complementing the YCF1 (vacuolar ABC type transporter)-depleting yeast mutant DTY167 with an expression library from Nicotiana tabacum. When NtPCS1 was over-expressed in DTY165 (WT) and DTY167 (mutant), tolerance to and the accumulation of cadmium (Cd) were enhanced. Interestingly, its expression promoted these responses as well to arsenic (As), but only in DTY167. We conclude that NtPCS1 plays a role in tolerance to and the accumulation of both toxic metals in Saccharomyces cerevisiae.
 
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accumulation;arsenite;cadmium;Nicotiana tabacum phytochelatin synthase;Saccharomyces cerevisiae;tolerance;
 
Journal of Plant Biology / v.48, no.4, 2005³â, pp.440-447
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ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200509408764328)
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