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한국식물학회 / v.48, no.4, 2005년, pp.483-486 
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( Endogenous Level of 28-Norcastasterone Is Strictly Regulated in Plant Cells ) |
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| 초 록 |
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| A cell-free enzyme solution prepared from cultured cells of Phaseolus vulgaris mediated C-24 methylation of 28-norcastasterone to castasterone with the aid of S-adenosylmethionine as a co-substrate in the presence of the NADPH cofactor. This enzyme solution also catalyzed conversion of 28-norcastasterone to a de methylated 28-norcastasterone, most likely 26,28-didemethyl-castasterone, when S-adenosylmethionine was not added to the enzyme solution. Furthermore, gene expression of Arabidopsis CYP85A1 and CYP85A2 mediating the conversion of 6-deoxo-28-norcastasterone to 28-norcastasterone was strongly inhibited by treatment of 28-norcastasterone. These results suggest that 28norcastasterone, along with castasterone and brassinolide, is an important brassinosteroid whose endogenous level should be strictly controlled to express brassinosteroid activities in plants. |
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| 키워드 |
| Arabidopsis;brassinosteroid;homeostatic regulation;28-norcastasterone;Phaseolus vulgaris; |
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Journal of Plant Biology / v.48, no.4, 2005년, pp.483-486
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200509408764683)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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