|
|
|
한국식물학회 / v.49, no.1, 2006년, pp.70-79 
|
( Carbon-Partitioning in Arabidopsis Is Regulated by the Fructose 6-Phosphate, 2-Kinase/Fructose 2,6-Bisphosphatase Enzyme ) |
| ;;;;;;; ;;;;;;;
|
|
|
 |
|
| |
| 초 록 |
|
|
| To further elucidate the mechanisms underlying carbon-partitioning in plants, we established an experimental system by generating transgenic Arabidopsis lines that overexpress both the fructose 6-phosphate, 2-kinase (F6P,2-K) and the fructose 2,6-bisphosphatase (F26BPase) domains. We also produced knockout transgenic plants for these domains via RNAi and T-DNA tagging. In F6p,2-K overexpressing transgenics, F6p'2-K activity increased slightly and $Fru-2,6-P_2$ levels were elevated by $80%$, compared with the wild type (WT). F26BPase activity was similar between the WT and transgenic plants. However, when that domain was overexpressed, F26BPase activity was increased by $70%$ compared with the WT, whereas F6p,2-K activity was reduced to $85%$ of the WT level. In knockout and RNAi mutant lines that showed reduced F6p,2-K and F26BPase activities, levels of $Fru-2,6-P_2$ were only between 3 to $7%$ of those for the WT: In F6p,2-K overexpressing transgenic lines, the levels of starch, hexose, and triose phosphates slightly increased, while sucrose content was marginally reduced. In F26BPase overexpressing plants, however, the levels of soluble sugars and hexose phosphates were slightly increased, but starch and triose phosphate contents declined. Furthermore, compared with the wr, the levels of soluble sugars rose while starch and hexose phosphate quantities decreased in 2-kinase/fructose-2,6-bisphophatase knockout mutants. Therefore, our data reaffirms that $Fru-2,6-P_2$ contributes to the regulation of photosynthetic carbon-partitioning between starch and sucrose in Arabidopsis leaves by limiting sucrose synthesis. |
| |
| 키워드 |
| Arabidopsis;carbohydrate metabolites;carbon-partitioning, fructose 2,6-bisphosphate;fructose 6-phosphate;2-kinase/fructose 2,6-bisphosphatase; |
| |
|
|
|
|
Journal of Plant Biology / v.49, no.1, 2006년, pp.70-79
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200614222987347)
언어 : 영어 |
|
| 논문 제공 : KISTI 한국과학기술정보연구원 |
|
|
|
|
|
|
