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한국식물학회 / v.49, no.2, 2006년, pp.133-140 
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( Identification of Differentially Expressed Genes Involved in Spine Formation on Seeds of Daucus carota L. (Carrot), Using Annealing Control Primer (ACP) System ) |
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| 초 록 |
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| Carrot seeds normally have surface spines. The availability of a spineless mutant would be agronomically beneficial, eliminating the current efforts to remove those spines pre-sowing. Furthermore, the identification of spine-specific genes would provide insights into spine development in wild-type carrot seed. This effort could be facilitated through the use of an annealing control primer (ACP) system. Here, we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) that involves ACPs for identifying genes of interest. With these techniques, 11 expressed sequence tags (ESTs) were obtained for cloning and sequencing the genes that are differentially expressed in wild-type spiny seeds, but not in the spineless mutant. In all, 7 cDNAs exhibited significant sequence similarity with known genes from other species. These included cell wall-associated hydrolase, tail fiber assembly protein, transcriptional regulatory protein, berberine bridge enzyme, S-adenosyl methionine synthase, transketolase, and phenylalanyl t-RNA synthetase beta chain. Four other cDNA sequences had no significant identities with known genes. As revealed by RT-PCR, these genes regulate spine formation during the developmental stage. Our results suggest that PCR-based differential display RT-PCR techniques are a very useful tool for identifying spine-specific genes from carrot seeds. |
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| 키워드 |
| annealing control primer (ACP);carrot;differentially expressed gene (DEG);RT-PCR;spineless;spiny; |
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Journal of Plant Biology / v.49, no.2, 2006년, pp.133-140
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200624718266620)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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