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한국식물학회 / v.49, no.4, 2006년, pp.303-308 
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( A 273-bp Promoter Region Is Responsible for Circadian Regulation of S-Adenosylmethionine Decarboxylase Gene Expression in Carnation ) |
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| 초 록 |
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| S-Adenosylmethionine decarboxylase (SAMDC) activity and mRNA amount increase in a light-dependent manner, and the accumulation of SAMDC transcripts might be partially regulated by a circadian clock in tritordeum and Pharbitis nil. How-ever, no research has been reported on the circadian regulation of SAMDC activity and corresponding gene expression. Here, we determined that SAMDC activity and its mRNA accumulation changed diurnally in carnation leaves. To identify the promoter region responsible for this rhythmicity, we performed northern blot analysis using a GUS cDNA probe in. transgenic tobacco plants. The GUS gene was under the control of serial deletions of the 5'-flanking region of a genomic clone corresponding to CSDC9 (gCSDC9). The region between -754 and -274, which negatively regulated the accumulation of GUS mRNA, had two HD-Zip-2 binding sequences (TAATAATTA) that were found by PLACE analysis of the promoter region. Another region at 273bp upstream from the transcription initiation site was sufficient for diurnal expression of GUS, and contained the putative sites responsible for diurnal expression, i.e., the GT-1 consensus sequence (GGTAAT) and a sequence necessary for this circadian expression (CAACTTCATC). |
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| 키워드 |
| S-adenosylmethionine decarboxylase;carnation;circadian clock;cis-element;promoter;transcriptional regulation; |
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Journal of Plant Biology / v.49, no.4, 2006년, pp.303-308
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200634741565393)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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