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한국식물학회 / v.49, no.4, 2006년, pp.291-297 
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( Ethylene-Regulated Expression of ACC Oxidase and ACC Synthase Genes in Mung Bean Hypocotyls ) |
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| 초 록 |
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| Ethylene induces an increase in transcript levels of the ACC oxidase gene (VR-ACS1) but suppresses expression of the ACC synthase gene (VR-ACS1) in etiolated mung bean hypocotyls. Here, treatment with either the transcription inhibitor ${alpha}-amanitin$ or the protein synthesis inhibitor cycloheximide (CHI) completely abolished ethylene-induced accumulation of VR-ACO1 mRNA. This indicated that ethylene-induction of VR-ACO1 is due to transcriptional activation, which also relies on de novo protein synthesis. In contrast, CHI induced the accumulation of VR-ACS1 transcripts.. ABA also inhibited ethylene-induced VR-ACO1 expression, but restored ethylene-suppressed VR-ACS1 expression. Results of time-course experiments and an interaction analysis of CHI and ABA suggested that the latter may exert its effect by preventing the synthesis of a factor(s) necessary for ethylene action. Ethylene-signaling was studied in more detail, using two pharmacological. inhibitors--EGTA and sodium orthovanadate. Those experiments demonstrated that calcium ions and a Tyr type of protein phosphatase may be involved in regulating ethylene biosynthetic genes. |
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| 키워드 |
| ABA;ACC oxidase;ACC synthase;calcium;ethylene;mung bean; |
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Journal of Plant Biology / v.49, no.4, 2006년, pp.291-297
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200634741565303)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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