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한국식물학회 / v.49, no.4, 2006년, pp.298-302 
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( Analysis of Gene Expression upon DNA Damage in Arabidopsis ) |
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| 초 록 |
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| To understand their responses to DNA damage, Arabidopsis plants were exposed to methyl methanesulfonate(MMS). Such treatment inhibited growth and decreased the leaf chlorophyll content. A concomitant change in the expression of cell cyclerelated genes also occurred. CYCB1 expression was slightly increased but that of CYCD1 declined. To identify the genetic elements in these responses, genome-wide transcription profiling of Arabidopsis was performed following the MMS treatment, using a cDNA microarray. Expression was altered by more than two-fold for 3666 genes - i.e., 1657 genes showed an increase and 2009 genes had a decrease in transcripts. Five DNA repair-related genes were slightly enhanced, while several disease resistance-related and glutathione transferase genes were strongly up-regulated. Interestingly, 27 ubiquitin-related genes were also altered by more than two-fold, suggesting that protein degradation may have been involved in those damage responses. These microarray results were validated by RT-PCR. |
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| 키워드 |
| Arabidopsis;DNA damage;methyl methanesulfonate;microarray; |
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Journal of Plant Biology / v.49, no.4, 2006년, pp.298-302
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200634741565348)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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