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한국식물학회 / v.49, no.4, 2006년, pp.281-285 
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( Recombinant AtNF-YA, a Subunit of the Arabidopsis CCAAT-Binding Transcription Factor, Forms a Protein-DNA Complex with Mammalian NF-YB and NF-YC ) |
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| 초 록 |
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| The evolutionarily conserved CCAAT binding protein NF-Y is a common regulatory DNA binding protein consisting of three distinct subunits. Unlike yeast and mammals, in which only a single copy of each subunit is encoded, Arabidopsis encodes a multi-gene family for each subunit in its genome. Compared with the NF-Y of mammals or yeast, very little is known about plant NF-Y homologs. Here Arabidopsis NF-YA subunits were isolated to determine whether they could form a heterotrimeric NF-Y complex with mammalian NF-YB and NF-YC. This resultant chimeric NF-Y complex had DNA binding ability to the same CCAAT sequences as those of the other life systems. Therefore, it is possible that plant NF-Y homologs might have biochemical characteristics similar to mammalian NF-Y, thereby suggesting its functional conservation among organisms. |
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| 키워드 |
| Arabidopsis genome;electrophoretic mobility shift assay;Nuclear Factor Y;yeast three-hybrid assay; |
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Journal of Plant Biology / v.49, no.4, 2006년, pp.281-285
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200634741565213)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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