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한국식물학회 / v.49, no.5, 2006년, pp.348-352 
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( Optimization of Direct Somatic Embryogenesis from Mature Zygotic Embryos of Panax ginseng C.A. Meyer ) |
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| 초 록 |
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| Culture conditions were optimized for somatic embryogenesis of Panax ginseng. The highest frequency of embryo formation was obtained when tissues were excised from the middle region of the cotyledon segments of zygotic embryos. Only treatment with light could stimulate the formation of single-type somatic embryos, whereas multiple-type somatic embryos and calli were observed under dark conditions. The highest production of somatic embryos was found with an $NH_4^+:NO_3{^-}$ ratio of 21:39. Among the tested media (MS, B5, and SH), maximum formation of somatic embryos was obtained when cotyledon explants were cultured on an 1% agar MS medium supplemented with 5% sucrose. Regenerated ginseng plantlets were transferred to an autoclaved soil mixture in the greenhouse. These transformants showed no detectable variations in their morphology or growth characteristics compared with the donor plant. |
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| 키워드 |
| agar;macro salts;Panax ginseng;somatic embryogenesis;sucrose; |
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Journal of Plant Biology / v.49, no.5, 2006년, pp.348-352
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200604623633498)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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