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한국식물학회 / v.49, no.5, 2006년, pp.358-363 
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( Increased Ethylene and Decreased Phenolic Compounds Stimulate Somatic Embryo Regeneration in Leaf Thin Section Cultures of Doritaenopsis Hybrid ) |
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| 초 록 |
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| The effects of endogenous levels of ethylene and phenolic compounds on somatic embryogenesis, medium-browning, and peroxidase activity were evaluated in thin section cultures of Doritaenopsis. Cultures were maintained for 8 weeks with four different treatments: i) thick leaf segment culture, ii) thin leaf section culture, iii) thin leaf section culture with ventilation, or iv) thin leaf section culture after explants were first washed. Explants cultured in closed vessels produced a larger number of somatic embryos than those reared in the ventilated vessels. This enhanced formation confirmed the greater involvement of accumulated ethylene under non-ventilated conditions, because wound-induced tissues from thin leaf sections normally release high level of ethylene. When explants were washed in the liquid medium and inoculated on the same solid medium, somatic embryo production was 1.7 and 18.5 times higher than in the thin section cultures and thick segment cultures, respectively. Reducing the level of phenolics in explants at the initial stage of culturing apparently stimulated this embryo regeneration. |
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| 키워드 |
| Doritaenopsis;ethylene;peroxidase activity;phenolic compounds;somatic embryo;thin section culture; |
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Journal of Plant Biology / v.49, no.5, 2006년, pp.358-363
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200604623633595)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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