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한국식물학회 / v.49, no.6, 2006년, pp.448-454 
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( Determination of Self-Incompatibility Genotypes of Korean Apple Cultivars Based on S-RNase PCR ) |
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| 초 록 |
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| To prevent self-fertilization, apple has a gametophytic self-incompatibility mechanism, part of a widespread intraspecific system, that is controlled by a multi-allelic locus. This attribute has been exploited in breeding programs for new cultivars. Likewise, many apple orchards depend on artificial pollination. Therefore, molecular analysis and early identification of the self-incompatibility (S) genotype could greatly improve breeding schemes and pollen donors selection. Here, we PCR-amplified the S-RNase PCR fragments from a total of 14 cultivars and parents, using new primers (ASPF3+ASPR3) common to 23 Salleles in apple. The S-genotypes were determined for the following: 'Hongro' $(S_1S_3)$, 'Gamhong' $(S_1S_9)$, 'Saenara' $(S_1S_3)$, 'Chukwang' $(S_3S_9)$, 'Hwahong' $(S_3S_9)$, 'Seokwang' $(S_3S_5)$, 'Hwarang' $(S_1S_9)$, 'Sunhong' $(S_3S_9)$, 'S.E.B.' $(S_1S_{19})$' 'S.G.D.' $(S_2S_3)$, and 'Mollie's Delicious' $(S_3S_7)$. We also confirmed the characteristics of the S-genotypes for eight Korean apple cultivars by PCR-Southern blot analysis, using seven S-RNases as probes. |
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| 키워드 |
| apple;gametophytic self-incompatibility;PCR;S-genotype;S-RNase; |
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Journal of Plant Biology / v.49, no.6, 2006년, pp.448-454
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200606141787324)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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