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한국식물학회 / v.50, no.2, 2007년, pp.161-166 
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( Dominance Relationship between Two Self-Incompatible Brassica campestris Haplotypes in Response to $CO_2$ Gas ) |
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| 초 록 |
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| In Brassica, self-incompatibility (SI) can be overcome by $CO_2$ application, an effective method for obtaining numerous inbred lines for $F_1$ commercial seed. We previously reported two different S-alleles of Brassica campestris, $S^{733};and;S^{734}$, with extremely different degrees of susceptibility to this gas. In the current study, we raised a cross-population between those two genetic lines, and analyzed their reaction level of self-incompatibility to $CO_2(RLSICO_2)$. Here, all 40 of our progeny from the $F_1$ cross-population were susceptible, maintaining high values of $RLSICO_2$. This suggests that the susceptible line, $S^{734}$, is dominant to the insusceptible line, $S^{733}$. We also generated an $F_2$ selfing-population of each crossed progeny, $S^{733}female;S^{734}male;and;S^{733}maleS^{734}female$, to assess the $RLSICO_2$ of each individual. PCR-RFLP analysis was performed to determine the S-genotype of the $F_2$ population. The $S^{734}$ allele segregated in a theoretical ratio of the dominant trait, and the $RLSICO_2$ was consistent with the dominance relationship. Therefore, we have now demonstrated that high $RLSICO_2$ in B. campestris is controlled by a dominant gene. |
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| 키워드 |
| dominant;PCR-RFLP;reaction level of self-incompatibility;S locus receptor kinase; |
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Journal of Plant Biology / v.50, no.2, 2007년, pp.161-166
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200717317898582)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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