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한국식물학회 / v.50, no.3, 2007년, pp.315-320 
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( In Vitro Biosynthesis of Strictosidine Using Lonicera japonica Leaf Extracts and Recombinant Yeast ) |
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| 초 록 |
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| Strictosidine is a key intermediate in the biosynthesis of the terpenoid indole alkaloid (TIA) pathway. It results from a condensation reaction, catalyzed by strictosidine synthase (STR), between tryptamine and secologanin. We have now developed a useful method, based on enzyme-assisted synthesis, to produce strictosidine. Our procedure utilizes leaf extracts from Japanese honeysuckle Lonicera japonica Thunb. as a secologanin source. In these experiments, an enzyme extract was prepared from transgenic yeast Saccharomyces cerevisiae that expresses the Catharanthus roseus STR (CrSTR) coding region. Strictosidine was then isolated with a 38% yield based on the initial amount of tryptamine in the enzymatic reaction. |
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| 키워드 |
| Catharanthus roseus;honeysuckle Lonicera japonica;secologanin;strictosidine;strictosidine synthase; |
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Journal of Plant Biology / v.50, no.3, 2007년, pp.315-320
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200734513388033)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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