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한국식물학회 / v.50, no.3, 2007년, pp.374-377 
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( High-Frequency in Vitro Flowering in Six Species of Ceropegia ) |
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| 초 록 |
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| High-frequency in vitro flowering is reported here from in vitro regenerated shoots of in vitro-raised seedlings of rare and endemic Ceropegia lawii, Ceropegia maccannii, Ceropegia oculata, and Ceropegia sahyadrica, as well as the widely distributed Ceropegia bulbosa var. bulbosa and Ceropegia hirsuta. In our first set of experiments, the MS medium contained 87 mM sucrose and was supplemented with varying concentrations of BAP (4.4 to $26.6;{mu}M$). For the second set of trials, varying concentrations of sucrose (87 to 233 mM) were tested in MS media containing a constant $4.4;{mu}M$ BAP. Sub-cultured apical as well as axillary buds flowered with similar frequencies after 30 d of incubation. For all six species, the highest percentage of flowering shoots was obtained with either $26.6;{mu}M$ BAP or 175 mM sucrose. Although smaller in size, their in vitro flowers were morphologically comparable with in vivo-derived flowers. Variations among species were noted for the number of flower buds per shoot and the percentage of flower formation. Because all six species showed similar responses in both experiments, we can suggest that this protocol is applicable across the wide range of Ceropegia species. |
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| 키워드 |
| Asclepiadaceae;BAP;biodiversity hotspot;rare and endemic;sucrose; |
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Journal of Plant Biology / v.50, no.3, 2007년, pp.374-377
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200734513388536)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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