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Çѱ¹½Ä¹°ÇÐȸ / v.50, no.5, 2007³â, pp.573-576
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( Biomass Production of Anoectochilus formosanus Hayata in a Bioreactor System ) |
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We investigated the factors that affect biomass production from Anoectochilus formosanus in a bioreactor system. Those factors included inoculum size, initial sucrose concentration, media supplements, photosynthetic photon flux density (PPFD), and culturing methods. An inoculum size of $8;g;L^{-1}$ was most suitable for shoot proliferation; biomass accumulation was optimized when the medium was supplemented with 3% sucrose compared with sucrose-free media or those containing concentrations of 6% or 9%. This accumulation also was enhanced under a PPFD of $50;{mu}mol;m^{-2};s^{-1}$. Likewise, the addition of coconut water ($50;mL;L^{-1}$) plus activated charcoal ($0.5;mg;L^{-1}$) to our Hyponex medium proved most beneficial. Comparative studies among three bioreactor systems - continuous immersion, raft (net), and temporary immersion (the ebb and flood system) - revealed that shoot proliferation and biomass accumulation were more efficient when culturing was performed under continuous immersion. |
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Anoechtochilus formosanus;bioreactor culture;jewel orchid; |
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Journal of Plant Biology / v.50, no.5, 2007³â, pp.573-576
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200703534302589)
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³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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