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한국식물학회 / v.50, no.5, 2007년, pp.557-561 
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( Isolation of New Microsatellite-containing Sequences in Acanthopanax senticosus ) |
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| 초 록 |
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| Microsatellite markers, also called simple sequence repeats (SSRs), are comprised of a 2- to 6-nucleotide repeat motif. They are useful as molecular markers for genetic authentication, crop breeding programs, and linkage analysis for map-based cloning. From a microsatellite-enriched genomic library of Acanthopanax senticosus, we identified 239 new microsatellite-containing sequences. The di-nucleotide repeat units were the most abundant (55.2%), followed by tri-nucleotide repeat units (24.6%). In detailed repeat structures, the $(AG)_n$ motif was most frequent (30.5%), followed by the $(AC)_n$ motif (21.7%). Hepta- and octa-nucleotide repeat motifs were found in each single locus, and a total of 33 (13.8%) complex repeat structures were recorded. This is the first report of mass isolation of microsatellites via screening of an A. senticosus library, and may well provide information useful as a genetic resource for the further study of A. senticosus. |
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| 키워드 |
| Acanthopanax senticosus;genetic marker;microsatellites;simple sequence repeats (SSRs); |
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Journal of Plant Biology / v.50, no.5, 2007년, pp.557-561
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200703534302505)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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