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한국식물학회 / v.50, no.6, 2007년, pp.681-686 
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( Rapid Shoot Propagation from Micro-Cross Sections of Kiwifruit (Actinidia deliciosa cv. 'Hayward') ) |
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| 초 록 |
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| Kiwifruit shoots can be rapidly propagated through a micro-cross section (MCS) system we established here. Optimal culture conditions were determined for different explant types, section sizes, and concentrations of inorganic salts and plant growth regulators. Rates of survival and callus formation were higher in half-strength MS salts than in full-strength MS media. Similar performance (i.e., survival and callus formation) was achieved with section sizes of either $800{mu}m$ or $1200{mu}m$. Proliferation efficiency was greatest when explants from stem tissue were cultured on 1/2 MS supplemented with $4.5{ imes}10^{-3}{mu}M$ 2,4-dichlo-rophenoxyacetic acid and $4.6{ imes}10^{-1}{mu}M$ zeatin. The number of shoots averaged 2.61 per explant, representing an efficiency of 94%. RAPD analysis revealed that the regenerated plants from our MCS system were genetically stable. These results show that the culturing of micro-cross sections from stem tissue is a powerful method for kiwifruit propagation. |
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| 키워드 |
| kiwifruit;normal shoots;section sizes;tissue types;vibratory microtome; |
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Journal of Plant Biology / v.50, no.6, 2007년, pp.681-686
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200706414177571)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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