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Çѱ¹½Ä¹°ÇÐȸ / v.51, no.1, 2008³â, pp.25-34
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( Overexpression of a Thellungiella halophila CBL9 Homolog, ThCBL9, Confers Salt and Osmotic Tolerances in Transgenic Arabidopsis thaliana ) |
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The calcineurin B-like (CBL) proteins, comprising a large subfamily of calcium sensors in plant cells, play an important role in many stress responses. We cloned a gene from the halophyte Thellungiella halophila that is homologous to AtCBL9 in Arabi-dopsis thaliana. The 1008-bp ThCBL9 contains an ORF of 639 bp and encodes 213 amino acids, with a 5'-untranslated region of 193 bp and a 3'-untranslated region of 176 bp. Its amino acid sequence shares high homology with AtCBLs. ThCBL9 is up regulated by ABA, NaCl, and PEG in Thellungiella leaves. Using molecular biological methods, we over-expressed ThCBL9 in A. thaliana and found that this enhanced tolerances to both high salt and osmotic stress in transgenic Arabidopsis. |
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overexpression;stress tolerance;ThCBL9;Thellungiella halophila; |
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Journal of Plant Biology / v.51, no.1, 2008³â, pp.25-34
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200810737042185)
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³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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