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Çѱ¹½Ä¹°ÇÐȸ / v.37, no.1, 1994³â, pp.101-109
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ChlamydomonasÀÇ Polyamine ÇÔ·®º¯È¿Í ¿±·Ïü DNA Methylation
( Changes in Polyamine Level and Chloroplast DNA Methylation in Chlamydomonas reinhardtii ) |
| À̼øÈñ; ¿¬¼¼´ëÇб³ À̰ú´ëÇÐ »ý¹°Çаú;
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| Chlamydomonas reinhardtii ¹è¿ìü À¯µµ±â µ¿¾È¿¡ polyamine »ýÇÕ¼º ¾ïÁ¦Á¦ÀÎ MGBG[methylglyoxal bis-(guanylhydrazone); SAMDC ¾ïÁ¦Á¦] 1mM 󸮱¸¿Í Á¤»ó ´ëÁ¶±¸¿¡¼ÀÇ polyamine ÇÔ·® º¯È¿Í ChlamydomonasÀÇ ct-DNA methylation°úÀÇ »óÈ£ ¿¬°ü¼ºÀ» Á¶»çÇÑ °á°ú, Àü¹ÝÀûÀ¸·Î 137C(£«)¿Í 137C(-)ÀÇ Á¤»ó ´ëÁ¶±¸¿¡¼ polyamine ¼öÁØÀº ¹è¿ìü À¯µµ±â Àü¹Ý¿¡ °ÉÃļ °¨¼ÒÃß¼¼·Î ³ªÅ¸³µÀ¸³ª, ƯÈ÷ 137C(£«)°¡ 137C(-)º¸´Ù polyamine ÇÔ·®ÀÌ ¸¹¾ÒÀ¸¸ç µ¿½Ã¿¡ °¨¼Ò·®µµ ÄÇ´Ù. 1mM MGBG 󸮱¸¿¡¼´Â 137C(£«)ÀÇ spermidine ÇÔ·®ÀÌ Á¤»ó ´ëÁ¶±¸º¸´Ù Áõ°¡ÇÏ¿´´Ù. In vitro¿¡¼ ctDNA methylation¿¡ ´ëÇÑ MGBGÀÇ ¿µÇâÀº Á¤»ó ´ëÁ¶±¸¿¡¼º¸´Ùµµ 1mM MGBG 󸮱¸¿¡¼ ¾à 20-30%ÀÇ ctDNA methylation °¨¼Ò¸¦ ³ªÅ¸³»¾ú´Ù. ¶ÇÇÑ, MGBG´Â in vitro¿¡¼ DNA methylase¿¡ ´ëÇÏ¿© ¾ïÁ¦È¿°ú¸¦ ³ªÅ¸³»¾îTEk. |
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| Relationship between polyamine level and DNA methylation in the absence or presence of MGBG(l mM), which is an enzyme-activated reversible inhibitor of SAMDC, has been investigated during gametogenesis of Chlamydomonas. In the absence of MGBG, polyamine levels decreased in Chlamydomonas 137C(+) and 137C(-) during gametogenesis. And polyamine level of 137C(+) was 2-5 times as much as that of 137C(-) and showed a significant decrease unlike that of 137C(-). In vitro, MGBG inhibited ctDNA methylation of 137C(+) by 20-30% but did not inhibited that of 137C(-). Also, MGBG inhibited DNA methylase by 60% in vitro. The results obtained in the present work suggest the possibility that the changes of polyamine level may be associated with ctDNA methylation during gametogenesis of Chlamydomonas.omonas. |
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| Chlamydomonas;polymine;MGBG;DNA methylasc;SAM decarboxylase; |
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Journal of Plant Biology / v.37, no.1, 1994³â, pp.101-109
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199411920117423)
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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