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Çѱ¹½Ä¹°ÇÐȸ / v.37, no.2, 1994³â, pp.195-201
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Methylglyoxal bis-(Guanylhydrazone)°ú PolymineÀÌ ´ëµÎ ÀÚ¿± ºÎÁ¤±Ù Çü¼º½Ã ź¼öȹ° ´ë»ç¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Effects of Methylglyoxal bis-(Guanylhydrazone) and Polyamines on Carbohydrate Metabolism during Adventitious Root Formation in Soybean cotyledons ) |
| ÇÑÅÂÁø; ÇѸ²´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;
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| Methylglyoxal bis-(guanylhydrazone)[MGBG]°ú polyamineÀÌ ºÎÁ¤±Ù Çü¼º½Ã ź¼öȹ° ´ë»ç¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» ¾Ë¾Æº¸°íÀÚ ´ëµÎ ÀÚ¿± ÀýÆíÀ» ÀÌ¿ëÇÏ¿© $5{ imes}10^{-6};M;NAA¿Í;5{ imes}10^{-7}M$ kinetinÀ¸·Î Á¶¼ºÇÑ ºÎÁ¤±Ù Çü¼º±¸¿¡ $10^{-3}M$ MGBG¿Í ÀÌ MGBG¿¡ $10^{-5}M$ÀÇ putrescine, spermidine ¹× spermineÀ» °¢°¢ È¥ÇÕ Ã³¸®ÇÏ¿© ÀüºÐ ¹× ¼ö¿ë¼º´çÀÇ ÇÔ·® º¯È¿Í ÇÔ²² °ü·Ã È¿¼ÒÀÇ È°¼ºµµ º¯È¸¦ Á¶»çÇÏ¿´´Ù. ÀüºÐ ¹× maltose¿Í sucrose ÇÔ·®Àº ´ëÁ¶±¸°¡ °¡Àå ³·¾ÒÀ¸¸ç, MGBG¸¦ Æ÷ÇÔÇÏ´Â ¸ðµç 󸮱¸°¡ ´ëü·Î ³ô°Ô ³ªÅ¸³µ´Ù. Glucose ÇÔ·® º¯È´Â ½ÃÂ÷´Â ÀÖÀ¸³ª ´ëÁ¶±¸¿Í spermine µ¿½Ã 󸮱¸°¡ À¯»çÇÏ¿© Áõ°¡ ÈÄ °¨¼ÒÇÏ¿´À¸¸ç MGBG 󸮱¸´Â °¨¼Ò ÈÄ Áõ°¡ÇÏ¿´´Ù. AmylaseÀÇ È°¼ºÀº ´ëÁ¶±¸¿Í MGBG 󸮱¸¿¡¼ À¯»çÇÑ ¾ç»óÀ» ³ªÅ¸³»¾î Áö¼ÓÀûÀ¸·Î Áõ°¡ÇÏ¿´À¸¸ç maltaseÀÇ È°¼ºÀº ´ëÁ¶±¸¿¡¼ °¡Àå ³ô¾Ò°í invertaseÀÇ È°¼ºÀº Å« º¯È°¡ ¾ø¾ú´Ù. |
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| In order to study the effects of polyamine inhibitors and polyamines on adventitious root formation, the correlation between adventitious root formation and carbohydrate metabolism was investigated in inoculated soybean (Glycine max L.) cotyledons at the concentration of $10^{-3};M$ methylglyoxal bis-(guanylhydrazone)[MGBG], and $10^{-3};M$ MGBG plus $10^{-5};M$ polyamines (putrescine, spermidine and spermine), respectively, for the adventitious root formation medium. The contents of starch, maltose and sucrose were lower in control and were higher in the treatments containing $10^{-3};M$ MGBG, and $10^{-3};M$ MGBG plus $10^{-5};M$ polyamines during culture. It was shown that the soluble sugar levels, except glucose, were higher than that of control in most of the treatments and the change in glucose contents tended to be similar to that control. The amylase activity increased in control and MGBG treatment, the maltase activity was higher in control and the invertase activity showed less substantial changes during culture.ulture. |
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| ºÎÁ¤±Ù;´ëµÎ;ź¼öȹ°;adventitious root;polyamines;MGBG;Glycine max;carbohydrates; |
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Journal of Plant Biology / v.37, no.2, 1994³â, pp.195-201
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199411920117168)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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