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한국식물학회 / v.37, no.2, 1994년, pp.231-236 
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시금치 엽록체의 광합성 전자전달 활성에 미치는 $Cd^{2+}$의 저해 효과
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| 정화숙; 경북대학교 사범대학 생물교육과;
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| 초 록 |
| 분리한 시금치 엽록체에 $Cd^{2+}$을 처리하였을 때 광계 I에 비해서 광계 II의 활성이 낮은 농도의 $Cd^{2+}$에 의해 억제되었다. 그러므로 $Cd^{2+}$에 의해 1차적으로 광계 II 활성이 저해되었다는 것을 알 수 있다. 광계 II의 전자공여체인 diphenylcarbazide를 첨가했을 때 대조구에 비해 활성이 크게 증가하였다. $Cd^{2+}$ 처리시 $Mn^{2+}과;Ca^{2+}을$ 외부에서 첨가했을 때 광계 II 활성은 회복되었지만 회복되는 정도가 15%로 낮았다. $50;mu extrm{M};Cd^{2+}$ 처리구에서 광계 II 활성은 크게 감소하였으나 Fv/Fm는 미소하게 감소하였으며 $100;mu extrm{M};Cd^{2+}$ 처리구에서 Fv/Fm는 크게 감소하였다. 광계 I활성은 $Cd^{2+}$의 처리 농도가 증가함에 따라 감소하였으나 세척하였을 경우 0.2 mM의 $Cd^{2+}$ 처리구에서 대조구보다 활성이 증가하였으며 농도가 높아짐에 따라 활성의 회복 정도가 컸다. |
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| The effect of $Cd^{2+}$ on the electron transport rate of PSI and PSII was investigated in isolated spinach chloroplasts. In photosystem II, the rate of electron transport was decreased as the concentration of $Cd^{2+}$ was increased from 1 to $100;mu extrm{M}$. The inhibitory effect of $Cd^{2+}$ was reduced when diphenylcarbazide was added to the reaction medium, indicating that $Cd^{2+}$ affects primarily psn oxygen evolving complexes of thylakoid membrane. The inhibitory effect of $Cd^{2+}$ was reduced when $Mn^{2+};and;Ca^{2+}$ were added to the reaction medium, but the inhibitory effect was not fully relieved. Although the activity of psn was decreased significantly by the treatment of $50;mu extrm{M};Cd^{2+}$, Fv/Fm was decreased slightly. However, the treatment of $100;mu extrm{M};Cd^{2+}$ resulted in the marked decrease of Fv/Fm. In photosystem I, the rate of electron transport decreased as the concentration of $Cd^{2+}$ was increased from 0.2 to 3.2 mM. The inhibitory effect of $Cd^{2+}$ was decreased when the chloroplast treated with $Cd^{2+}$ was washed by centrifugation.gation. |
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| 키워드 |
| 시금치 엽록체;광계 I;광계 II;엽록소 형광;spinach chloropast;$Cd^{2+}$;PSI;PSII;chlorophyll fluorescence; |
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Journal of Plant Biology / v.37, no.2, 1994년, pp.231-236
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199411920117301)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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