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Çѱ¹½Ä¹°ÇÐȸ / v.37, no.3, 1994³â, pp.387-394
º¸¸®¼ö³ª¹« À¯½Ä¹°ÀÇ »ýÀå°ú Áú¼Ò°íÁ¤ Ȱ¼º¿¡ ´ëÇÑ È¯°æ¿äÀÎÀÇ ¿µÇâ
( Effects of Environmental Factors on Growth and Nitrogen Fixation Activity of Autumn Olive (Elaeognus umbellata) Seedlings )
¼Û½Â´Þ; °æºÏ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;
 
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º¸¸®¼ö³ª¹«(Elaeagus umbellata Thunb.) À¯½Ä¹°ÀÇ »ýÀ°°úÁ¤¿¡¼­ ±Ù·ù Çü¼º ¹× Áú¼Ò°íÁ¤ Ȱ¼º¿¡ ´ëÇÑ Áß¿ä ¿äÀÎÀ¸·Î¼­ ±¤µµ, ¿Âµµ, Áú¼ÒÈ­ÇÕ¹°, ¼öºÐ ½ºÆ®·¹½º µîÀÇ ¿µÇâÀ» Á¤·®ÀûÀ¸·Î ºÐ¼®ÇÏ¿´´Ù. À¯½Ä¹°ÀÇ »ó´ë»ýÀå·ü°ú T/Rºñ´Â °¢°¢ ÃʱâÀÇ $1.60%{cdot}d^{-1}$¿Í 1.12¿¡¼­ ±Ù·ù Çü¼ºÀÌ ÃËÁøµÈ Èı⿡´Â $3.75%{cdot}d^{-1}$¿Í 2.31·Î Áõ°¡ÇÏ¿´´Ù. Áú¼Ò°íÁ¤ÀÇ ºñȰ¼ºÀº ±Ù·ù Çü¼º Ãʱ⿡ $72.5;mu extrm{M};C_2H_4{cdot}g;fr;wt;nodule^{-1}{cdot}h^{-1}$·Î¼­ ÃÖ´ëÄ¡¸¦ ³ªÅ¸³½ ÈÄ Á¡Â÷ °¨¼ÒÇÏ¿´´Ù. »ó´ëÁ¶µµ 20-25. 10-15 ¹× 4-6% 󸮱¸ÀÇ Áú¼Ò°íÁ¤ Ȱ¼ºÀº ´ëÁ¶±¸¿¡ ´ëÇØ °¢°¢ 41, 54 ¹× 71%ÀÇ °¨¼Ò¸¦ º¸¿´´Ù. ±Ù·ù¸¦ 15, 20, 25 ¹× $30^{circ}C$¿¡¼­ ¹è¾çÇÒ ¶§ Ȱ¼ºÀº °¢°¢ 5.4, 24.7, 51.6 ¹× $58.5;mu extrm{M};C_2H_4{cdot}g;fr;wt;nodule^{-1}{cdot}h^{-1}$·Î º¯ÇÏ¿´´Ù. Àú¿Â($15^{circ}C$)ÀÇ Àüó¸® ÈÄ $30^{circ}C$·Î ¿Å°åÀ» ¶§ ±Ù·ù Ȱ¼º($66.5;mu extrm{M};C_2H_4{cdot}g;fr;wt;nodule^{-1}{cdot}h^{-1}$)Àº °í¿Â($35^{circ}C$) Àü󸮿¡ ºñÇØ ÃËÁøµÇ¾ú´Ù. ±Ù·ùÀÇ È°¼ºÀº »ê¼ÒºÐ¾Ð¿¡ ºñ·ÊÇÏ¿© 16 kPa À̻󿡼­ Æ÷È­µÇ¾ú°í, ±Ù·ù¸¦ »Ñ¸®¿¡¼­ ¶¼¾î³»°Å³ª Àý´ÜÇϸé 92%¿Í 43%·Î Ȱ¼ºÀÌ ¾ïÁ¦µÇ¾ú´Ù. pH 5.5, 6.5 ¹× 8.0¿¡¼­ »ýÀ°µÈ Ãʱ⠱ٷùÀÇ »ó´ëȰ¼ºÀº °¢°¢ 89, 100 ¹× 40%¿´À¸¸ç, 1 ¹× 3 mMÀÇ $NO_3;-N¿Í;{NH_4}^{+}-N$¿¡¼­ »ýÀ°µÈ Ãʱ⠱ٷù´Â °¢°¢ 6.1%¿Í 68.50%ÀÇ »ó´ëȰ¼ºÀ» º¸¿´´Ù. ¼öºÐ °ø±Þ·® 20, 50 ¹× 100mL¿¡ ÀÇÇÑ ±Ù·ùÀÇ Çü¼º°ú ºñȰ¼ºÀº °¢°¢ 35, 120 ¹× 8 mg.plant-1¿Í 33.6, 58.4 ¹× $8.4;mu extrm{M};C_2H_4{cdot}g;fr;wt;nodule^{-1}{cdot}h^{-1}$¿´´Ù. ½Ä¹°Ã¼¿¡ ¼öºÐ°ø±ÞÀ» Áß´ÜÇÏ¿´À» ¶§ 5ÀÏ ÈÄ¿¡ ÀϽà À§Á¶°¡ ³ªÅ¸³µÀ¸¸ç, ½Ä¹°Ã¼ÀÇ ÇÔ¼ö·® ¹× Áõ»ê·®Àº °¢°¢ 90 ¹× 53% °¨¼ÒµÇ¾úÀ¸³ª, ±Ù·ù Ȱ¼ºÀº ¿ÏÀüÈ÷ ¼Ò½ÇµÇ¾ú´Ù.
Effects of environmental factors of light, temperature, nitrogen sources and water stress were analyzed quantitatively on the nodule formation and nitrogen fixation activity of autumn olive plant (Elaeagnu$ umbellala Thunb.) during the seedling growth. Seedlings showed the maximum nitrogenase activity of $72.5;mu extrm{M};C_2H_4{cdot}g;fr;wt;nodule^{-1}{cdot}h^{-1}$ in the early nodulation stage. The relative growth rate and T/R ratio changed from $1.60%{cdot}d^{-1}$ and 1.12 in the earlier stage to $3.75%{cdot}d^{-1}$ and 2.31 in the later stage, respectively. light conditions of 20-25, 1015 and 4-6% resulted in decreases of 41, 54 and 71% of the nitrogenase activity, respectively. Nodules incubated in 15, 20, 25 and $30^{circ}C$ showed the activities of 5.4, 24.7, 51.6 and $58.5;mu extrm{M};C_2H_4{cdot}g;fr;wt;nodule^{-1}{cdot}h^{-1}$ respectively. Pretreatment with low temperature ($15^{circ}C$) followed incubation at $30^{circ}C$ attained higher nitrogenase activity ($66.5;mu extrm{M};C_2H_4{cdot}g;fr;wt;nodule^{-1}{cdot}h^{-1}$) than that with higher temperature ($35^{circ}C$). The oxygen pressure above 16 kPa is necessary for saturation of the nodule activity, but the activity was inhibited severely by physical impact such as the exision or isolation of nodules from the root. The relative activities of early nodules grown in pH 5.5, 6.5 and 8.0 were 89, 100 and 40% and those grown in 1 and 3 mM of $NO_3;and;NH_4$ were 6, 1 and 68, 50%, respectively. Watering levels of 20, 50 and 100 mL during the seedling growth resulted in 35, 120 and 8 mg of nodule formation and 33.6, 58.4 and $8.4;mu extrm{M};C_2H_4{cdot}g;fr;wt;nodule^{-1}{cdot}h^{-1}$ of the nitrogenase activity, respectively. Water stress with 86% decrease of soil water content caused temporary wilting point of leaf and a complete disappearance of nitrogenase activity of nodules, though the water content and transpiration rate in plant were reduced to 90 and 53%, respectively.tively.
 
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º¸¸®¼ö³ª¹«;À¯½Ä¹° »ýÀå;Áú¼Ò°íÁ¤ Ȱ¼º;ȯ°æ¿äÀÎ;Elaeagnus umbellata;seedling growth;nitrogen fixation;environmental factor;
 
Journal of Plant Biology / v.37, no.3, 1994³â, pp.387-394
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199411920117091)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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